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Anti-alpha Tubulin antibody - Microtubule Marker
Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
(1)
Write a review
Citations:
(308)
Supplier Page
Supplier Page from
Abcam for
Anti-alpha Tubulin antibody - Microtubule Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-alpha Tubulin antibody - Microtubule Marker
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab18251
Antibody Type
Primary
Quantity
50 UG
Isotype
IgG
Host
Rabbit
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Clonality
Polyclonal
Conjugate/Tag
Unconjugated
Concentration
0.3 mg/ml
Gene Name
TUBA4A
Reactivity
Human, Mouse, Rat
Target
alpha Tubulin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (308)
(1)
Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury
Neural regeneration research
December 1, 2023
Ma C, Wang J, Tu Q, Bo W, Hu Z, Zhuo R, Wu R, Dong Z, Qiang L, Liu Y,
et al
.
minutes or 2 hours. Primary antibodies were as follows: anti-α-tubulin (rabbit,
1:1200
,
Abcam
, Cat#
ab18251
, RRID: AB_2210057), anti-tyrosinated tubulin (
rat
,
1:800
, Abcam, Cat#
ab6160
, RRID: AB_305328), anti-acetylated
(2)
O‐GlcNAcylation Regulates Centrosome Behavior and Cell Polarity to Reduce Pulmonary Fibrosis and Maintain the Epithelial Phenotype
Advanced Science
November 14, 2023
Fan Yu, Song Yang, Hua Ni, Dai Heng, Xuemei Wu, Mulin Yang, Xinming Zhang, Yuxin Cao, Yandong Pei, Di An,
et al.
anti‐E‐cadherin (610 182, BD); anti‐PARD6B (
sc166405
,
Santa Cruz Biotechnology
, Dallas, TX); anti‐α‐tubulin (
ab18251
,
Abcam
); anti‐γ‐tubulin (
ab11316
,
Abcam
); anti‐β‐actin (
ab8226
,
Abcam
); anti‐GFP (11 814 460 001, Roche,
(3)
Phosphorylation controls spatial and temporal activities of motor‐PRC1 complexes to complete mitosis
The EMBO Journal
November 2, 2023
Agata Gluszek‐Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie PI Welburn
tubulin (
1:1,000
anti‐beta tubulin,
mouse
,
T7816
,
Sigma
OR
1:2,000
/
1:1,000
anti‐alpha tubulin, rabbit,
ab18251
,
Abcam
) and
PRC1
(
sc‐376983
,
Santa Cruz
,
1:200
mouse
; McHugh et al, 2018). Anti‐CENP‐E (
Abcam
,
Ab5093
;
(4)
Cross-protection and cross-neutralization capacity of ancestral and VOC-matched SARS-CoV-2 adenoviral vector-based vaccines
npj Vaccines
October 4, 2023
Sabrina E. Vinzón, María V. Lopez, Eduardo G. A. Cafferata, Ariadna S. Soto, Paula M. Berguer, Luciana Vazquez, Leonora Nusblat, Andrea V. Pontoriero, Eduardo M. Belotti, Natalia R. Salvetti,
et al.
and probed with an anti-spike Ab (
40150-T62
,
Sino Biological
Wayne, PA, USA) and anti-α-tubulin Ab (
Ab18251
;
Abcam
, Cambridge, UK). Spike expression was detected and quantified by
densitometry
using the ImageJ
(5)
High-sensitive nascent transcript sequencing reveals BRD4-specific control of widespread enhancer and target gene transcription
Nat Commun
August 17, 2023
Annkatrin Bressin, Olga Jasnovidova, Mirjam Arnold, Elisabeth Altendorfer, Filip Trajkovski, Thomas A. Kratz, Joanna E. Handzlik, Denes Hnisz, Andreas Mayer
(Cell Signaling Technology, Cat.# C29F4,
1:1000
), H3K27ac (
Abcam
,
ab4729
,
1:1000
), Tubulin (
Abcam
,
ab18251
,
1:3000
), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (
LiCOR
, Cat.#
926–32210
,
1:15,000
), IRDye®
(6)
NPC1 plays a role in the trafficking of specific cargo to melanosomes
Journal of Biological Chemistry
August 1, 2023
Alina Adriana Rus, Ioana V. Militaru, Ioana Popa, Cristian V.A. Munteanu, Livia Elena Sima, Nick Platt, Frances M. Platt, Ștefana M. Petrescu
anti-
NPC1
(
ab108921
), mouse anti-MITF (
ab80651
), rabbit anti-Calnexin (
ab22595
) and rabbit anti-tubulin (
ab18251
) antibodies were purchased from
Abcam
.
Mouse
anti-HMB-45 (M06340-1) antibody was from Agilent.
LysoTracker Green (DND-26
(7)
Super-resolution imaging unveils the self-replication of tau aggregates upon seeding
Cell reports
July 25, 2023
Dimou E, Katsinelos T, Meisl G, Tuck BJ, Keeling S, Smith AE, Hidari E, Lam JYL, Burke M, Lövestam S,
et al
.
RRID:AB_10013724"
Mouse
MC1 antibody,Peter Davies Lab,RRID:AB_2314773 Rabbit anti-alpha tubulin,
Abcam
,"Cat#
ab18251
, RRID:AB_2210057"
Mouse
anti-alpha tubulin,DHSB,"Cat# 12G10, RRID:AB_2315509" Rabbit anti-ubiquitin
(8)
Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset
eLife
July 11, 2023
Mansur A, Joseph R, Kim ES, Jean-Beltran PM, Udeshi ND, Pearce C, Jiang H, Iwase R, Milev MP, Almousa HA,
et al
.
Anti-KBTBD5
for
KLHL40
,
1:100
dilution (
sc-99943
,
Santa Cruz Biotechnology
); anti-α-Tubulin,
1:500
(
ab18251
,
Abcam
); anti-
Sar1a
,
1:100
(
ab125871
,
Abcam
);
anti-Sec24d
,
1:100
(
14687
,
Cell Signaling technology
);
(9)
Coordination of actin plus-end dynamics by IQGAP1, formin, and capping protein
bioRxiv
July 6, 2023
Morgan L. Pimm, Alexandra G. Marcin, Brian K. Haarer, Marcela Alcaide Eligio, Jessica L. Henty-Ridilla
anti-IQGAP1 (
1:1000
;
610612
,
BD Biosciences
, East Rutherford, NJ), polyclonal rabbit α-tubulin (
1:2500
;
ab18251
,
Abcam
Inc, Cambridge, United Kingdom), and appropriate secondary antibodies (
1:5000
IR-dye-680 for
(10)
Live-Cell SOFI Correlation with SMLM and AFM Imaging
ACS Bio & Med Chem Au
June 21, 2023
Riley B. Hargreaves, Sam Duwé, Ashley M. Rozario, Alison M. Funston, Rico F. Tabor, Peter Dedecker, Donna R. Whelan, Toby D. M. Bell
SMLM. Single color
microtubule
imaging used rabbit anti-α-tubulin 1° antibody (
1:500
in 5% BSA,
Abcam
ab18251
) and Alexa Fluor 647 goat anti-rabbit IgG 2° antibody (
1:200
in 5% BSA,
Thermo Fisher
A-21245
). For
(11)
Cortical dynein drives centrosome clustering in cells with centrosome amplification
Molecular Biology of the Cell
May 15, 2023
Dayna L. Mercadante, William A. Aaron, Sarah D. Olson, Amity L. Manning
morphology, cells were fixed in ice-cold methanol for 10 min at –20°C. Blocking, primary (α-tubulin:
Abcam
ab18251
, Cambridge, UK; centrin: Millipore
04-1624
, Burlington, MA) and secondary antibody dilutions were prepared
(12)
Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patterns
The EMBO Journal
May 15, 2023
Leonid Serebreni, Lisa‐Marie Pleyer, Vanja Haberle, Oliver Hendy, Anna Vlasova, Vincent Loubiere, Filip Nemčko, Katharina Bergauer, Elisabeth Roitinger, Karl Mechtler,
et al.
anti‐FLAG,
Sigma‐Aldrich
,Cat#
F3165
Secondary anti‐mouse HRP,
Sigma‐Aldrich
,Cat#
12‐349
Histone H3
,
Abcam
,Cat#
ab1791
Alpha‐tubulin,
Abcam
,Cat#
Ab18251
Secondary anti‐rabbit HRP,
Sigma‐Aldrich
,Cat#
12‐348
Oligonucleotides and other sequence‐based reagents
(13)
Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro
Stem cell reviews and reports
May 1, 2023
Yu X, Wang N, Wang X, Ren H, Zhang Y, Zhang Y, Qiu Y, Wang H, Wang G, Pei X,
et al
.
32,894, Santa Cruz), c-KIT (
1:500
,
ab5505
,
Abcam
), CX37 (
1:300
,
ab83788
,
Abcam
), and α-Tubulin (
1:400
,
ab18251
,
Abcam
) were used. The same batch of differentiated cells was treated with isotype-matched IgG and used
(14)
Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies
Frontiers in Pharmacology
April 24, 2023
Khalid Alghamdi, Zeyad Alehaideb, Ashok Kumar, Hamad Al-Eidi, Sahar S. Alghamdi, Rasha Suliman, Rizwan Ali, Feras Almourfi, Saleh M. Alghamdi, Mohamed Boudjelal,
et al.
MITF
.
Mouse
monoclonal (
clone
236–10501, #
A11126
,
Thermo Fisher Scientific
) and rabbit polyclonal (#
ab18251
,
Abcam
, Cambridge, MA) anti-α tubulin antibodies were loading controls. Infrared fluorescent dye IRDye®
(15)
Heat denaturation enables multicolor X10-STED microscopy
Scientific reports
April 1, 2023
Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO.
#
T6199
Sigma-Aldrich
, #
302211
Synaptic Systems
, Göttingen, Germany, #
302203
Synaptic Systems
, and #
ab18251
Abcam
, Cambridge, UK), using AbberiorStar635P (AS635P) labeled secondary nanobodies (#N1202-Ab635P-S
(16)
Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity
Nature communications
March 27, 2023
He S, Gillies JP, Zang JL, Córdoba-Beldad CM, Yamamoto I, Fujiwara Y, Grantham J, DeSantis ME, Shibuya H.
GR3251355-4),
KIF11
(
Eg5
) (
1:100
for IF;
Sigma
;
HPA010568
, A118067), α-tubulin (
1:1000
for IF;
Abcam
;
ab18251
, GR3406015-1), SYCP1 (
1:1000
for IF;
Abcam
;
ab15090
, GR3184119-1),
NuMA
(
1:100
for IF;
Abcam
;
ab36999
,
(17)
Selectivity of Hydroxamate- and Difluoromethyloxadiazole-Based Inhibitors of Histone Deacetylase 6 In Vitro and in Cells
International Journal of Molecular Sciences
March 1, 2023
Jakub Ptacek, Ivan Snajdr, Jiri Schimer, Zsofia Kutil, Jana Mikesova, Petra Baranova, Barbora Havlinova, Werner Tueckmantel, Pavel Majer, Alan Kozikowski,
et al.
5% BSA in TBS at 4 °C overnight. Antibodies used were anti-alpha tubulin (
1:4000
dilution, rabbit, #
Ab18251
,
Abcam
, Cambridge, UK), anti-acetylated tubulin (
1:2500
dilution,
mouse
, #
T7451
,
Sigma-Aldrich
, St.
(18)
Overexpression of a Novel Noxo1 Mutant Increases Ros Production and Noxo1 Relocalisation
International Journal of Molecular Sciences
February 28, 2023
Fatima-Zahra Benssouina, Fabrice Parat, Claude Villard, Ludovic Leloup, Françoise Garrouste, Jean-marc Sabatier, Lotfi Ferhat, Hervé Kovacic
Enzo Life Sciences
,1/400, Anti-Vimentin,Rabbit,R28
3932
;
Cell Signaling
,2/200, Anti Alpha-tubulin,Rabbit,
Ab18251
;
Abcam
,1/800,
Anti-Keratin
kit,Rabbit and
Mouse
,
9384
;
Cell signaling
,, Anti Citrate synthase,
Mouse
,SC390693;
(19)
CAMSAP2 localizes to the Golgi in islet β-cells and facilitates Golgi-ER trafficking
iScience
February 17, 2023
Ho KH, Jayathilake A, Mahircan Y, Nour A, Osipovich AB, Magnuson MA, Gu G, Kaverina I.
used are as follows: anti-CAMSAP2 (
1:500
, #
NBP1-21402
, Novus Biologicals), anti-α-tubulin (
1:1000
, #
ab18251
,
Abcam
),
anti-GM130
(
1:200
, #
610823
, BD Biosciences), IRDye 800 goat anti-rabbit IgG (
1:5000
, Rockland
(20)
Systematic identification and characterization of repressive domains in Drosophila transcription factors
The EMBO Journal
February 1, 2023
Loni Klaus, Bernardo P de Almeida, Anna Vlasova, Filip Nemčko, Alexander Schleiffer, Katharina Bergauer, Lorena Hofbauer, Martina Rath, Alexander Stark
ChemiDoc MP imaging system
(
Bio‐Rad
). For a loading control, blots were probed with a primary
anti‐Tubulin
antibody (
Abcam
,
ab18251
).
Immunoprecipitation
‐
mass spectrometry
(IP‐MS) experiments RDs were cloned into the zfh1‐DSCP‐3xFLAG‐Gal4‐dpse‐EBFP2
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Reviews
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(1)
3/13/2017
Antibody Against Alpha Tubulin For Immunofluorescence
Adam Kramer
This antibody is great to image the microtubule cytoskeleton in cultured mammalian cells
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Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
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